A previously described canonical Treg signature32 was enriched in both tumor groups in comparison with the LV control group (67.8% in ES and 64.4% in AS), as shown by the significant expression of canonical Treg genes such as Foxp3, Il2ra, Nt5e, and Ebi3 (Supplementary Fig. 4a). This evidence concerns the gene EBI3 and neoplasm.