TGFB1 and neoplasm: Although activated host Tregs in tumor-bearing mice displayed an increased level of cell surface LAP (TGF-β complexed to latency-associated peptide) as compared to tumor-free mice, accompanied with GARP expression, TGF-β signaling was probably not involved in the de novo generation of tumor-specific Tregs or anergy induction (Supplementary Fig. 10a, b) since the administration of an anti-mouse TGF-β neutralizing antibody to mice bearing tumors did not modify Marilyn pTreg conversion or anergy induction (Supplementary Fig. 10c-e).