Next, we further examined whether miR-550a-3-5p downregulated endogenous YAP expression and found that miR-550a-3-5p overexpression was able to downregulate YAP mRNA and protein levels after a relatively short time (24 h) in HCT116 cells (Fig. 3d) and confirmed its YAP targeting in various types of cancer cells, including colon cancer (RKO and SW620 cells), head and neck cancer (Fadu and HEp-2 cells), lung cancer (H460 and A549 cells), and breast cancer (MCF7 and T47D cells) (Fig. 3e). This evidence concerns the gene YAP1 and malignant colon neoplasm.