In our investigation, we discovered that anti-apoptotic protein Bcl-2 was downregulated while the levels of pro-apoptotic protein Bax and cleaved Caspase-3 were increased significantly in HCT116 cells after administration of NKP608, which suggested that NKP608, at least partly, induced cell apoptosis in colorectal cancer cells. This evidence concerns the gene BAX and colorectal cancer.