CCND1 and cancer: Thus, to investigate potential mechanism of inhibitory cancer in colorectal cancer cells by NKP608, western blotting was applied to detect Wnt signaling relative members. As shown in Fig. 4, the results revealed that treatment with NKP608 significantly downregulated Wnt-3a and β-catenin, additionally, reduced Wnt downstream target molecules linked with cell proliferation such as Cyclin D1 and VEGF.