At baseline, multivariate regression analyses (Table 2) showed that plasma tHcy concentration was associated with age (p = 0.001), creatinine concentration (p = 0.001), prevalent CVD (3% lower in subjects with a history of unstable angina (p = 0.03) or 6% lower in subjects with a history of myocardial infarction (p = 0.001) compared with subjects with a history of ischemic stroke), and MTHFR genotype (19% higher in subjects with TT genotype compared with those with CC genotype, p = 0.001). This evidence concerns the gene MTHFR and ischemic stroke.