REM2 and infection: Acute cortical slices were then isolated either 4 days post infection (4 d.p.i., Figure 8A left) or 10–12 d.p.i. (Figure 8A right), and f-I curves constructed from GFP+, layer 2/3 pyramidal neurons to determine if Rem2 regulates neuronal intrinsic excitability in a cell-autonomous manner.