To test our hypothesis that miR‐21 mediates TGF‐β1 induced CMT by targeting Jagged1, first we detected the expression of Jagged1 in CFs treated with TGF‐β1, Ad‐miR‐21 sponge, or/and siJagged1. This evidence concerns the gene TGFB1 and Charcot-Marie-Tooth disease.