•     Pulse, blood pressure, respiratory rate and temperature measurements takenAbout 20mls mls blood sample taken for the following laboratory assays•     Haematology: Full Blood Count, screen for sickle cell trait.•     Biochemistry: Sodium, Potassium, Urea, Creatinine, Albumin, ALT and bilirubin.•     Diagnostic serology: HIV antibodies, Hepatitis B.•     Immunological assays of prior exposure to malaria•     Diagnostic Malaria TestsUrinalysis, and for women pregnancy testElectrocardiograms (ECGs) for evidence of heart disease.Medical and social history and clinical assessment. This evidence concerns the gene ALB and hepatitis B virus infection.