We cultured 3D tumorspheres of HuH7 in a serum-free NSA media (Figure 5A) and quantified the expression levels of HCC stemness markers CD44 (Figure 5B), CD133 (Figure 5C), CD90 (Figure 5D), and Oct4 (Figure 5E), as well as miRNA122a (Figure 5F). The gene discussed is POU5F1; the disease is hepatocellular carcinoma.