The ISH studies also suggested PTTG3P to be mainly localized to the cytoplasm of HCC tissues (Fig. 1d).The level of PTTG3P in seven HCC cell lines (HepG2, Huh7, MHCC-97H, SMMC-7721, QGY7701, SK-Hep1, Hep3B) and hepatic immortal cell line LO2 was also detected by qRT-PCR (Additional file 3: Figure S1b). This evidence concerns the gene PTTG3P and hepatocellular carcinoma.