We obtained and selected Hca-P-ANXA5-shRNA1, Hca-P-ANXA5-shRNA2, and Hca-P- ANXA5-shControl monoclonal cell lines, by G418 screening combined with limited dilution method, to study ANXA5 dynamic change on HCC progression and metastasis. Here, ANXA5 is linked to hepatocellular carcinoma.