Replication of both X4 and R5 variants of HIV in primary CD4 + T cells was also inhibited by treatment with the bryologs (Fig. 3) in a short-term 3 day spreading infection assay, further supporting the possibility that the same PKC activating agents that induce HIV from latency could also help contain the spread of that virus to uninfected cells. This evidence concerns the gene CD4 and infection.