AGT and hypertensive disorder: We induced hypertension in C57Bl/6 mice by infusion of Ang II (490 ng/kg/min) for 6 days and analysed single cell suspensions of the kidney, aorta, spleen, and periaortic lymph nodes for the presence of monocytes, macrophages (MΦ) and DCs using a gating strategy that effectively allows discrimination of these cells (Figure 7A).