The aim of this study is to investigate the potential inhibitory effect of α-chaconine and α-solanine to clarify the potential of inhibiting proliferation, and to explain the effect of these glycoalkaloids on the Akt signaling and estrogen receptor α (ERα) in RL95-2, which is estrogen receptor (ER) positive human endometrial cancer cell line. Here, ESR1 is linked to endometrial cancer.