On the other hand, the immunohistochemical investigation also revealed that the very weak expression of CLIC1 was associated with lymphatic metastasis, the 5-year overall survival rate was significantly poorer in the very weak CLICI expression group than in the other group, and the very weak expression of CLIC1 was an independent prognostic factor in human ESCC tissues. The gene discussed is CLIC1; the disease is esophageal squamous cell carcinoma.