PKD1 protein expression was then analyzed by western blot in three cell lines showing PRKD1 mRNA expression (MDA-MB-436, CAMA-1, HCC-38) and in three cell lines in which PRKD1 transcript was not detected (MDA-MB-231, MDA-MB-468, HCC-1937). The gene discussed is PKD1; the disease is hepatocellular carcinoma.