We compared relative level of phosphorylated Stat1 (Tyr701) and phosphorylated Stat3 (Tyr705) proteins and observed combination of HER2 and HER3 knock down with Th1 cytokines significantly increased ratio of phosphorylated Stat1:Stat3 (1:1.9) compared to untreated cells (p = 0.0001) and Th1 cytokine treatment alone (Figure 5B, pStat1:pStat3 = 1:1.2, p = 0.0042), indicating reversal of Stat phosphorylation and subsequent senescence and apoptosis effects on cancer cells. This evidence concerns the gene STAT3 and cancer.