One, while they used only ischemic insult, we used a triple method (hypoglycemia, tMCAO, and tPA) to injure the BBB, and two, while transgenic HSP27 was overexpressed before the ischemic insult in Leak’s studies, we injected HSP27 after the ischemic and hyperglycemic insults, which is closer to the situation that would be experienced by stroke patients. This evidence concerns the gene PLAT and Stroke.