Apoptosis induced by siFAM188B was not limited to HCT-116 cells because compared with NC siRNA-treated cells, siFAM188B treatment also increased annexin V/PI-positive cells in other colon cancer cell lines, HT-29 and SW620 (Fig. 2f, Supplementary Figures 3d and 4d). Here, ANXA5 is linked to malignant colon neoplasm.