Almost all TNBC cells expressed activated Notch1 and/or Notch3 protein (N3IC), thus confirming the hyperactivation of Notch signaling observed in this BC subtype14, mainly involving the upregulation of N3IC expression, as it appears at undetectable levels in MCF10A, a normal immortalized mammary epithelial cell line. The gene discussed is NOTCH3; the disease is breast cancer.