AQP4 and Hypoglycemia: To determine whether the knockdown of AQP4 in astrocytes was capable of preventing BBB disruption triggered by hypoglycemia in an indirect way, we set up non-contact co-cultures of astrocytes without AQP4 and bEnd.3 cells and assessed transendothelial permeability by measuring the transendothelial transport of Na-F 12 or 24 h after treatment with 0 mM glucose.