A reduced titration of MBNL1 by expanded CCUG repeats is consistent with the lesser splicing and differentiation defects reported in DM2 muscle cell cultures compared to DM1 cells32–34, and with the lesser alternative splicing changes that we observed in DM2 distal muscles compared to DM1 distal muscle samples. The gene discussed is MBNL1; the disease is myotonic dystrophy type 1.