To determine whether NET-induced Mφ pyroptosis following sepsis is a specific receptor-dependent event, we isolated BMDM from WT, RAGE−/−, TLR4−/−, and TLR9−/− mice, and then treated the cells with NETs (or the medium supernatant of the unstimulated PMN as a control) for 12 h. This evidence concerns the gene TLR4 and Sepsis.