Two of these ANO5-KO mice, produced by disruption of either exon 118 or exon 219, do not exhibit any obvious muscle pathology; whereas the third line of ANO5-KO mice, produced by gene-trapping between exons 8 and 915, showed very mild myopathy as evidenced by a very small increase in serum CK and central nucleation. This evidence concerns the gene ANO5 and myopathy.