We then examined the mRNA expression of these genes in H1650 cells in comparison with that in a gefitinib-sensitive EGFR-mutant NSCLC cell line, HCC827, and found that RNF25 and EEF1D are expressed at more than 1.5-fold higher levels in H1650 than in HCC827 (Fig. 1b, Supplemental Fig. 1c). This evidence concerns the gene RNF25 and non-small cell lung carcinoma.