In six published LC cohorts (Garber Lung, Landi Lung, Su Lung, Hou Lung, Selamat Lung, and Okayama Lung), we observed significantly decreased PLAC8 expression of LC, but show no difference in Wachi Lung, which may have been caused by the smaller sample size (n = 10) (Fig. S1B). This evidence concerns the gene PLAC8 and laryngotracheoesophageal cleft.