In a study using 2D cultures of iPSCs derived from an 82-year-old sAD patient, researchers were able to achieve some key AD features in vitro, including formation of abnormally phosphorylated Tau protein, increased expression of glycogen synthase kinase-3β (the protein kinase that phosphorylates Tau) and up-regulation of genes linked to oxidative stress response [134]. The gene discussed is MAPT; the disease is Alzheimer disease.