Finally, we revealed a higher expression of Galectin-3 and TLR4 in lung cancer tissues; Galectin-3, TLR4 and NEAT1 was positively correlated with each other, indicating that Galectin-3/TLR4/NF-κB/NEAT1 path might be another contributor to the excessive cancer cell proliferation and migration of lung adenocarcinoma. Here, LGALS3 is linked to lung carcinoma.