Here, we explored the upstream regulatory factors and mechanisms of NEAT1 abnormal overexpression in lung adenocarcinoma, and further suggest a regulatory path formed by Galectin-3, TLR4, NF-κB and NEAT1 that may contribute to the hyperproliferation and migration of lung adenocarcinoma cells. The gene discussed is NFKB1; the disease is lung adenocarcinoma.