In addition to RNA-Seq experiments, we also used quantitative real-time PCR (qPCR) to analyze Etv5 RNA expression in independently-generated non-neoplastic (Nf1flox/flox [2]; abbreviated N for normal) and tumor-bearing (Nf1flox/null GFAP-Cre [2]; “OPG-1”) optic nerves. Here, GFAP is linked to neoplasm.