The aim of this project was to better understand the mechanism of the anticancer effects on grade IV glioma cells and U87MG cells after treatment of L. sibiricus transformed root extract (TR) and transgenic root extract with transcriptional factor AtPAP1; these effects may be facilitated by increased DNA damage, PARP cleavage, H2A.X histone and UHRF-1/DNMT1 regulation. Here, UHRF1 is linked to central nervous system cancer.