qPCR analysis showed that LPO exposure consistently stimulated the expression of multiple putatively causal genes in NAFLD pathological processes, including: fatty acid synthesis (ACACA, LXR, SREB1c), gluconeogenesis (PCK, G6PD) and lipid vesicular transport proteins Perilipin 1 and 2 (PLIN1, PLIN2) (figure 1g). This evidence concerns the gene ACACA and metabolic dysfunction-associated steatotic liver disease.