Our work aligns with two earlier findings: swimming exercise training, which positively regulated ACh-induced endothelial dysfunction in the aorta of ApoE KO25, and treadmill exercise, which prevented endothelial dysfunction with the increased expression of p-eNOS, NO production, and antioxidant protein in the thoracic aorta of LDL-deficient mice15. This evidence concerns the gene NOS3 and endothelial dysfunction.