RA-FLS receiving pCDNA3.1 or pCDNA3.1-SIRT1 transfection was harvested for Western blot of NF-κB family proteins, including NF-κB p65, phospho-NF-κB p65 (p-p65, Ser536), and acetyl-NF-κB p65 (Ac-p65, Lys310). The gene discussed is SIRT1; the disease is rheumatoid arthritis.