To test whether DARPP-32 requires downstream IKKα and NF-κB2 signaling to promote migration, we overexpressed DARPP-32 upon shRNA-mediated knockdown of IKKα or NF-ĸB2 in human NSCLC cells (Supplementary Fig. 9a, b). Here, PPP1R1B is linked to non-small cell lung carcinoma.