Since HBc assembles into capsid particles rapidly in hepatoma cells, which can affect CTD phosphorylation state indirectly by influencing the accessibility of the CTD phosphorylation sites to host kinases and phosphatases, and CTD also undergoes dynamic phosphorylation and dephosphorylation associated with pgRNA packaging and reverse transcription, we decided to use the RRL in vitro translation system for HBc expression and assembly that we developed recently [35]. This evidence concerns the gene KRT88P and hepatocellular carcinoma.