In addition we performed the same procedure using two osteosarcoma cell lines–U2OS (CC genotype) and MG63 (TT genotype) and the results showed that the demethylating agent increased OPTN and NF-κB target genes in U2OS while NF-κB expression was maintained (S2A Fig), suggesting that a change in the methylation status due to the effect of the demethylating agent had an effect in OPTN and in NF-κB target genes expression. The gene discussed is OPTN; the disease is osteosarcoma.