Using FRT/Flp-mediated locus-specific cassette exchange, we generated isogenic Jurkat leukemia cell lines allowing for Doxycycline (Dox)-inducible, single-copy expression of affinity-tagged variants of seven MLL-fusions that were previously proposed to employ different molecular mechanisms of oncogenic transformation (MLL-AF1p, MLL-AF4, MLL-AF9, MLL-CBP, MLL-EEN, MLL-ENL, MLL-GAS7, Fig. 1a, b and Supplementary Fig. 1a-c). Here, MLLT1 is linked to leukemia.