Tumor-bearing mice were then treated daily with I.V. injections of vehicle, JQ1, TMZ, or JQ1 + TMZ in free drug form, or drugs packaged in either non-functionalized PEG-NPs or Tf-NPs at equivalent doses of 2 mg kg−1 per drug for 5 days and tumor signal evaluated using luciferase bioluminescence. Here, TF is linked to neoplasm.