Consistent with our previous western blot results, immunohistochemical analysis showed that the expression levels of EGFR, Ki67, cyclin E, CDK4, p-AKT, p-mTOR, and p-P70S6K were significantly downregulated in the tumor tissues of miR-1231-treated nude mice compared with controls (Fig. 7d). Here, CDK4 is linked to neoplasm.