To examine this possibility, brachyury was overexpressed in parallel experiments in the colon carcinoma line, HCT116, which carries wild-type p53, and the non-small cell lung carcinoma (NSCLC) line, H1299, which express a non-functional isoform of p53 (Figures 2B,C). The gene discussed is TP53; the disease is non-small cell lung carcinoma.