To explore the biological impact of CDC73_3YF in AML cells, we transduced Cdc73fl/fl-CreERT2 mouse bone marrow with MLL-AF9 packaged retrovirus to generate stable AML cell lines that can be induced to genetically excise Cdc73 by treatment with 4-hydroxytamoxifen (4-OHT) (Figure 1B, Supplementary Figure 1C) [51]. Here, MLLT3 is linked to acute myeloid leukemia.