Western blots demonstrate that AML cells overexpressing SETDB1 have a marked increase in global H3K9me3 (Supplementary Figure 2B), however, neither loss of Cdc73 nor re-expression of CDC73_3YF affected the global levels of H3K9me3 (Supplementary Figure 2C). This evidence concerns the gene SETDB1 and acute myeloid leukemia.