AFP and hepatocellular carcinoma: However, when we considered HCC patients with AFP ≥60 mg/dl or AFP-L3 ≥20%, the expression frequency of one or more HPC markers (≥1 M) and two or more HPC markers (≥2 M) in HCC increased to 59.5% and 29.9% for AFP (Figure 7B) and 59.3% and 31.2% for AFP-L3, respectively (Figure 7C), and further increased to 67.9% and 45.3%, respectively, for patients with high serum levels of both AFP and AFP-L3 (Figure 7D).