The frequencies of a single marker expression were 10.4% (DLK1), 6.8% (EpCAM), 4.0% (NCAM), and 2.4% (CK19), indicating that other HPC marker-positive HCCs (approximately 33.7% of the total number of HPC marker-positive tumours) expressed two or more HPC proteins. The gene discussed is NCAM1; the disease is neoplasm.