Given the accumulated evidence that Bregs are also potent regulators of colitis (60–64), that the deficiency of Bregs in mice results in exacerbated arthritis with increased frequency of TH17 cells and decreased Foxp3+ Tregs (65), we have now tested the efficacy of AS-ODN BM-DC to treat IBD using the dextran sodium sulfate (DSS) colitis mouse model and to determine the degree of Treg and/or Breg involvement. This evidence concerns the gene FOXP3 and colitis.