In order to analyze the effects of the designed CRISPR/Cas9 construct to HIV-1 regulatory protein expression, we first developed stable, FLAG-bound Tat- and Rev-expressing HEK 293 T cell lines by infecting cells with a lentiviral vector bearing FLAG-tagged HIV-1 NL4-3 tat and rev, mimicking actual HIV-1 infection. Here, TAT is linked to HIV-1 infection.