HAMP and hepatocellular carcinoma: To determine whether the TFR2 alpha and HAMP promoters were methylated in Huh7 hepatoma cells, and to confirm their methylation status in HepG2 cells, we carried out bisulphite conversion of DNA and used specific primers to interrogate the CpGs within each promoter that corresponded to the 450K probe sites (Illumina Infinium HumanMethylation450K BeadChip) from the ENCODE project.