This study established rat models of middle cerebral artery occlusion (MCAO) injecting with EGCG using arterial pump, observed neurological function and brain injury, established neuronal cell injury model of cerebral ischemia and hypoxia, and investigated the regulatory mechanisms of PI3K/AKT/eNOS signaling pathway in vivo and in vitro. This evidence concerns the gene AKT1 and brain ischemia.