In this paper, we demonstrated that Kv1.3 channels, which are expressed in astrocytes in the glioma environment, modulate GLT-1 trafficking and functions: in particular, Kv1.3 inhibition decreases the desumoylation of the GLT-1 transporter, thereby promoting its membrane expression and, consequently, its activity. The gene discussed is SLC1A2; the disease is central nervous system cancer.