Additionally, miRNA-mRNA regulatory network analysis indicated that five down-regulated genes (Abcg8, Cyp1a1, Tmem255a, Gstm6 and Lat) in NAFLD rats were common targets of miR-200b-3p, miR-200b-5p and miR-200c-3p. Here, TMEM255A is linked to metabolic dysfunction-associated steatotic liver disease.