KRAS and pancreatic neoplasm: To test whether changes in ceramide expression levels occur, we probed the ceramide de novo synthesis pathway by analyzing the expression of enzymes SPT, CERS-6, DEGS1 at 2, 4, and 6 hours after dosing with GT3, K-Ras mutated MIA PaCa-2 (Fig. 1a-f) and Panc 1 (Fig. 2a-f), and wild type BxPC3 pancreatic cancer cells (Fig. 2g-l).