Both electrophoretic mobility shift assay (EMSA) (Figure 5C; Figure S5D) and immunofluorescence confocal microscopy of P65 nuclear translocation (Figure 5D; Figure S6) revealed increased nuclear abundance of NF-κB in TNF-treated RA-FLSs that were concomitantly treated with Torin-1. This evidence concerns the gene TNF and rheumatoid arthritis.