Regarding our choice of cell line, we engineered Ln229 glioblastoma cells that were endogenously mutant at the proline rich N-terminal domain (p53P98L, i.e. not affecting the DNA-binding domain), to express the biosensor containing one of four different p53 proteins: p53wt, p53Y220C, p53G245S and p53R282W. Here, TP53 is linked to glioblastoma.